The Native Cell Membrane Nanoparticle system: A comprehensive, detergent-free protein extraction, sample preparation, and structure elucidation platform for 
drug discovery. 

With the NCMN detergent-free extraction technique, cell membrane proteins retain more of their native lipid environments, enabling more accurate structure determinations with cryo-EM, NMR, EPR and MS workflows, and computational fitting techniques.

The NCMN system is available to research partners packaged in a complete kit for ease-of-use and consistent results, or as part of a complete, end-to-end cryo-EM structure determination workflow.

Collaboration and contract research

We currently offer the NCMN platform for Federal agency, academic research, and pharmaceutical contract research collaborations.

Advisory Board


Wayne Hendrickson
Columbia University

Violin Family Professor of Physiology and Cellular Biophysics

Chief Life Scientist in the Photon Sciences Directorate at Brookhaven National Laboratory

Scientific Director of the New York Structural Biology Center

National Academy of Sciences

Joachim Frank
Columbia University

Professor of Biochemistry and Molecular Biophysics

2017 Nobel Prize in Chemistry 

National Academy of Sciences

Research Partners

Guo Lab


NIH Grant 2020 RO1


cryo-EM facilities


Cryo-EM facilities


Sanket Deshmukh, Associate professor

Membrane proteins function naturally as imbedded in the lipid bilayers of cell membranes, but isolation into homogeneous and soluble preparations is needed for many biochemical studies. Detergents, which are used traditionally to extract and purify membrane proteins from cells, also remove most protein-associated lipid molecules as they disrupt the membranes. We have devised a detergent-free system to prepare native cell-membrane nanoparticles for biochemical analysis. In application to the membrane transporter AcrB, we demonstrate that these detergent-free nanoparticles are suitable for cryo-EM imaging at high resolution and that the natural lipid-bilayer structure so preserved is important for the functional integrity of AcrB. This nanoparticle system should be broadly applicable in membrane-protein research.

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